pFLAG-Myc-CMV-19 vector (V011135)

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LOCUS       pFLAG-Myc-CMV-19        4758 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expression and secretion of FLAG- and Myc-tagged proteins
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pFLAG-Myc-CMV-19
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Sigma-Aldrich
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             932..976
                     /label=preprotrypsin leader
                     /note="leader sequence from mouse preprotrypsin"
     CDS             977..1000
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    998..1060
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1061..1090
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     polyA_signal    1099..1721
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        1750..2079
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        complement(2118..2136)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2150..2166)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2174..2190)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2198..2228)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2243..2264)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2552..3140)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3314..4171)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4172..4276)
                     /label=AmpR promoter
     rep_origin      4303..4758
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"