pIRES vector (V011109)

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LOCUS       pIRES.        6105 bp DNA     circular SYN 01-JAN-1980
DEFINITION  IRES-containing vector for expressing two genes in mammalian cells 
            from the same bicistronic transcript.
ACCESSION   .
VERSION     .
KEYWORDS    pIRES
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1085..1107
                     /label=MCS A
                     /note="MCS A"
                     /note="multiple cloning site"
     misc_feature    1152..1738
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1737..1770
                     /label=MCS B
                     /note="MCS B"
                     /note="multiple cloning site"
     promoter        complement(1774..1791)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase
                     (shorter by one base than the standard T3 promoter)"
     polyA_signal    complement(1809..1930)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      2116..2571
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2688..3045
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3096..3887
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3954..4002
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        4308..4412
                     /label=AmpR promoter
     CDS             4413..5270
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5444..6032
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"