pSecTag A vector (V011087)

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LOCUS       pSecTag_A.        5166 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian expression vector for the secretion of C-terminally 
            6xHis-tagged proteins (replaced by pSecTag2 A). For other reading 
            frames, use pSecTag B or pSecTag C.
ACCESSION   .
VERSION     .
KEYWORDS    pSecTag A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     An in-frame stop codon is present near the end of the MCS.
FEATURES             Location/Qualifiers
     source          1..5166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     905..967
                     /label=Ig-kappa leader
                     /note="leader sequence from mouse immunoglobulin kappa 
                     light
                     chain"
     misc_feature    970..1088
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1089..1118
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1134..1151
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    1180..1404
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1450..1878
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1892..2221
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2269..2316
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2335..2706
                     /label=BleoR
                     /note="antibiotic-binding protein"
     polyA_signal    2839..2972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3009..3025)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3033..3049)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3057..3087)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3102..3123)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3411..3999)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4173..5030)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5031..5135)
                     /label=AmpR promoter