pT-REx-DEST31 vector (V011078)

LOCUS       pT-REx-DEST31.        7559 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) destination vector enabling N-terminal 6xHis-tagging and 
            tetracycline-inducible high-level expression.
ACCESSION   .
VERSION     .
KEYWORDS    pT-REx-DEST31
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7559)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        9..312
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        313..516
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    518..536
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    539..557
                     /gene="tetO"
                     /label=tetracycline repressor TetR binding site
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             661..663
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             673..690
                     /label=6xHis
                     /note="6xHis affinity tag"
     protein_bind    715..839
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        864..894
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             948..1604
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1949..2251
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2295..2419)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2480..2498)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2509..2525)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2930..3064
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      3190..3645
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3764..4121
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4172..4963
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5030..5078
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        5384..5488
                     /label=AmpR promoter
     CDS             5489..6346
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6520..7108
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"