Basic Vector Information
- Vector Name:
- pTRE-Dual1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3199 bp
- Type:
- Mammalian Expression Vectors
- Replication origin:
- ori
- Source/Author:
- Clontech
- Copy Number:
- High copy number
- Promoter:
- tight TRE
pTRE-Dual1 vector Map
pTRE-Dual1 vector Sequence
LOCUS pTRE-Dual1. 3199 bp DNA circular SYN 01-JAN-1980
DEFINITION Vector for co-expressing two genes with the Tet-On(R) Advanced or
Tet-Off(R) Advanced system.
ACCESSION .
VERSION .
KEYWORDS pTRE-Dual1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3199)
AUTHORS Clontech
TITLE Direct Submission
REFERENCE 2 (bases 1 to 3199)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3199
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 4..318
/label=tight TRE promoter
/note="Tet-responsive promoter PTight, consisting of seven
tet operator sequences followed by the minimal CMV
promoter"
misc_feature 323..366
/label=MCS 1
/note="MCS 1"
/note="multiple cloning site 1"
misc_feature 368..954
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 955..1005
/label=MCS 2
/note="MCS 2"
/note="multiple cloning site 2"
polyA_signal 1113..1194
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1376..1964)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2138..2995)
/label=AmpR
/note="beta-lactamase"
promoter complement(2996..3100)
/label=AmpR promoter
This page is informational only.