pTRE-Dual2 vector (V011061)

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LOCUS       pTRE-Dual2.        3884 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for co-expressing mCherry and another gene with the Tet-On(R)
            Advanced or Tet-Off(R) Advanced system.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE-Dual2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..318
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     CDS             335..1042
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    1053..1639
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1640..1690
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    1798..1879
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2061..2649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2823..3680)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3681..3785)
                     /label=AmpR promoter