pTRE-Tight-BI-ZsGreen1 vector (V011058)

MSDS

Basic Vector Information

Vector Name:: pTRE-Tight-BI-ZsGreen1

Antibiotic Resistance:: Ampicillin

Length:: 3509 bp

Type:: Mammalian Expression Vectors

Replication origin:: ori

Source/Author:: Clontech

Copy Number:: High copy number

Promoter:: tight TRE

pTRE-Tight-BI-ZsGreen1 vector Vector Map

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pTRE-Tight-BI-ZsGreen1 vector Sequence

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LOCUS       pTRE-Tight-BI-Zs        3509 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for co-expressing ZsGreen and another gene with the Tet-On(R)
            Advanced or Tet-Off(R) Advanced system.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE-Tight-BI-ZsGreen1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..318
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     misc_feature    335..405
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    519..600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(782..1370)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1544..2401)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2402..2506)
                     /label=AmpR promoter
     polyA_signal    complement(2539..2620)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2737..3429)
                     /label=ZsGreen
                     /note="Zoanthus green fluorescent protein (Matz et al.,
                     1999)"
     promoter        3440..3509
                     /label=bidirectional TRE promoter
                     /note="Tet-responsive bidirectional promoter PTight-BI, 
                     consisting of seven tet operator sequences flanked on each 
                     side by the minimal CMV promoter"

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