pTRE2hyg vector (V011054)

LOCUS       V011054                 5337 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011054
VERSION     V011054
KEYWORDS    pTRE2hyg
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    15..285
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     promoter        318..356
                     /label="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          554..1126
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             1130..1252
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    1323..1378
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     rep_origin      complement(1947..2535)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2709..3566)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3567..3671)
                     /label="AmpR promoter"
     polyA_signal    complement(3777..3858)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(3978..5000)
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(join(5009..5337,1))
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"