pTRE3G-BI-Het1 vector (V011052)

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LOCUS       pTRE3G-BI-Het1.        3524 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for doxycycline-inducible dual expression of FKBP- 
            and FRB-tagged proteins, allowing drug-inducible heterodimerization.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE3G-BI-Het1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..451
                     /label=TRE3G BI promoter
                     /note="3rd-generation Tet-responsive bidirectional promoter
                     that can be activated by binding of Tet-On(R) 3G"
     misc_feature    452..485
                     /label=5' MCS-1
                     /note="5' MCS-1"
                     /note="multiple cloning site upstream of DmrA"
     CDS             486..488
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             495..815
                     /label=FKBP
                     /note="human FK506-binding protein FKBP12"
     misc_feature    816..831
                     /label=3' MCS-1
                     /note="3' MCS-1"
                     /note="multiple cloning site downstream of DmrA"
     polyA_signal    967..1048
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1230..1818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1992..2849)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2850..2954)
                     /label=AmpR promoter
     polyA_signal    complement(2987..3068)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    3199..3210
                     /label=3' MCS-2
                     /note="3' MCS-2"
                     /note="multiple cloning site downstream of DmrC"
     CDS             complement(3211..3489)
                     /label=FRB
                     /note="FKBP-rapamycin binding domain of human FRAP"
     CDS             complement(3499..3501)
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     misc_feature    3502..3519
                     /label=5' MCS-2
                     /note="5' MCS-2"
                     /note="multiple cloning site upstream of DmrC"