pTRE3G-BI-mCherry vector (V011051)

MSDS

Basic Vector Information

Vector Name:: pTRE3G-BI-mCherry

Antibiotic Resistance:: Ampicillin

Length:: 3564 bp

Type:: Mammalian Expression Vectors

Replication origin:: ori

Source/Author:: Clontech

Copy Number:: High copy number

Promoter:: TRE3G BI

pTRE3G-BI-mCherry vector Vector Map

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pTRE3G-BI-mCherry vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pTRE3G-BI-mCherr        3564 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for doxycycline-inducible expression of a gene together with 
            the mCherry fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE3G-BI-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..451
                     /label=TRE3G BI promoter
                     /note="3rd-generation Tet-responsive bidirectional promoter
                     that can be activated by binding of Tet-On(R) 3G"
     misc_feature    452..511
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    625..706
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(888..1476)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1650..2507)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2508..2612)
                     /label=AmpR promoter
     polyA_signal    complement(2645..2726)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2843..3550)
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"

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