pTRE3G-BI vector (V011049)

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LOCUS       pTRE3G-BI.        2880 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for doxycycline-inducible expression of two genes in cells 
            containing the Tet-On(R) 3G transactivator protein.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE3G-BI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..451
                     /label=TRE3G BI promoter
                     /note="3rd-generation Tet-responsive bidirectional promoter
                     that can be activated by binding of Tet-On(R) 3G"
     misc_feature    452..511
                     /label=MCS 1
                     /note="MCS 1"
                     /note="multiple cloning site 1"
     polyA_signal    625..706
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(888..1476)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1650..2507)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2508..2612)
                     /label=AmpR promoter
     polyA_signal    complement(2645..2726)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2834..2875
                     /label=MCS 2
                     /note="MCS 2"
                     /note="multiple cloning site 2"