pTRE3G-ZsGreen1 vector (V011046)

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LOCUS       pTRE3G-ZsGreen1.        4700 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for doxycycline-inducible expression of a gene together with 
            the ZsGreen fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE3G-ZsGreen1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     This plasmid actually contains ZsGreen with its original codons 
            rather than the mammalian codon-optimized ZsGreen1.
FEATURES             Location/Qualifiers
     source          1..4700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..382
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     CDS             389..1081
                     /label=ZsGreen
                     /note="Zoanthus green fluorescent protein (Matz et al.,
                     1999)"
     misc_feature    1092..1679
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1686..1721
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     intron          1852..1917
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2047..2067
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2492..2613
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2870..3458)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3632..4489)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4490..4594)
                     /label=AmpR promoter