pZFHD1-2 vector (V011043)

LOCUS       40924_48383        3991 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector encoding a promoter that can be activated by 
            drug-induced heterodimerization of ZFHD1 and the p65 activation 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    88..297
                     /label=12  ZFHD1 binding sites
                     /bound_moiety="ZFHD1"
                     /note="ZFHD1 binding sites"
                     /note="12 binding sites for the composite human DNA-binding
                     domain ZFHD1 (Pomerantz et al., 1995)"
     promoter        320..433
                     /label=IL-2 promoter
                     /note="minimal promoter from the human interleukin-2 gene"
     misc_feature    462..505
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    619..740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(747..1202)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1229..1333
                     /label=AmpR promoter
     promoter        1335..1692
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1727..2518
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    2753..2800
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3129..3717
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    3779..3827
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3841..3932
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"