Basic Vector Information
- Vector Name:
- pET-21d(+)
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5440 bp
- Type:
- pET & Duet Vectors (Novagen)
- Replication origin:
- ori
- Source/Author:
- Novagen (EMD Millipore)
- Copy Number:
- High copy number
pET-21d(+) vector Map
pET-21d(+) vector Sequence
LOCUS pET-21d(+). 5440 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for inducible expression of N-terminally T7-tagged proteins. Same reading frame as pET-21c(+) but with an NcoI site at the start codon. ACCESSION . VERSION . KEYWORDS pET-21d(+) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5440) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 5440) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..5440 /mol_type="other DNA" /organism="synthetic DNA construct" terminator complement(26..73) /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(140..157) /label=6xHis /note="6xHis affinity tag" misc_feature 158..203 /label=MCS /note="MCS" /note="multiple cloning site" CDS complement(205..237) /label=T7 tag (gene 10 leader) /note="leader peptide from bacteriophage T7 gene 10" RBS complement(244..266) /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(281..305) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(306..324) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 633..710 /label=lacI promoter CDS 711..1790 /label=lacI /note="lac repressor" protein_bind 1806..1827 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS 2602..2790 /label=rop /note="Rop protein, which maintains plasmids at low copy number" misc_feature 2895..3037 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(3223..3811) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3985..4842) /label=AmpR /note="beta-lactamase" promoter complement(4843..4947) /label=AmpR promoter rep_origin complement(4974..5429) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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