Basic Vector Information
- Vector Name:
- pET-23(+)
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3592 bp
- Type:
- pET & Duet Vectors (Novagen)
- Replication origin:
- ori
- Source/Author:
- Novagen (EMD Millipore)
- Copy Number:
- High copy number
pET-23(+) vector Map
pET-23(+) vector Sequence
LOCUS pET-23(+). 3592 bp DNA circular SYN 01-JAN-1980
DEFINITION Bacterial vector for expression of genes with bacterial translation
signals.
ACCESSION .
VERSION .
KEYWORDS pET-23(+)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3592)
AUTHORS Novagen (EMD Millipore)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 3592)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3592
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator complement(26..73)
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(140..157)
/label=6xHis
/note="6xHis affinity tag"
misc_feature 158..203
/label=MCS
/note="MCS"
/note="multiple cloning site"
promoter complement(227..245)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 754..942
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
misc_feature 1047..1189
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(1375..1963)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2137..2994)
/label=AmpR
/note="beta-lactamase"
promoter complement(2995..3099)
/label=AmpR promoter
rep_origin complement(3126..3581)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
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