pET-25b(+) vector (V011008)

LOCUS       pET-25b(+).        5547 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector with a signal sequence for expression of 
            C-terminally HSV-tagged proteins in the periplasm.
ACCESSION   .
VERSION     .
KEYWORDS    pET-25b(+)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(140..157)
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             complement(164..196)
                     /label=HSV tag
                     /note="HSV (herpes simplex virus) epitope tag"
     misc_feature    212..279
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     sig_peptide     complement(278..343)
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
     RBS             complement(351..373)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(388..412)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(413..431)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        740..817
                     /label=lacI promoter
     CDS             818..1897
                     /label=lacI
                     /note="lac repressor"
     protein_bind    1913..1934
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             2709..2897
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    3002..3144
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3330..3918)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4092..4949)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4950..5054)
                     /label=AmpR promoter
     rep_origin      complement(5081..5536)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"