pGA643 vector (V010851)

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LOCUS       40924_20870       11601 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pGA643, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   An G, Ebert PR, Mitra A, Ha SB.
  TITLE     Binary Vectors
  JOURNAL   (in) Gelvin,S.B. and Schilperoort,R.A. (Eds.); PLANT MOLECULAR 
            BIOLOGY MANUAL: A3/1-19; Kluwer Academic Publishers (1988)
REFERENCE   2  (bases 1 to 11601)
  AUTHORS   Kim S-R., Kang H-G., Lee S, Kim K-M., An G.
  TITLE     Complete sequence of the pGA643 binary vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 11601)
  AUTHORS   Kim S-R., Kang H-G., Lee S, Kim K-M., An G.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2004) Division of Molecular and Life Science, 
            POSTECH, Nam-Gu Hyoja San31, Pohang, Kyungbuk 790-784, Republic of 
            Korea
REFERENCE   4  (bases 1 to 11601)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 11601)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "(in) 
            Gelvin,S.B. and Schilperoort,R.A. (Eds.)"; volume: " PLANT MOLECULAR
            BIOLOGY MANUAL"; pages: " A3/1-19; Kluwer Academic Publishers (1988"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (01-NOV-2004) Division of Molecular and Life Science, POSTECH, 
            Nam-Gu Hyoja San31, Pohang, Kyungbuk 790-784, Republic of Korea"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..25)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        181..364
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             365..1183
                     /codon_start=1
                     /label=KanR
                     /note="fusion between an N-terminal peptide of nopaline
                     synthase and Tn5 aminoglycoside phosphotransferase"
                     /translation="MAITLSATSLPISARIRAGSPAAWVERLFGYDWAQQTIGCSDAAV
                     FRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLG
                     EVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVD
                     QDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLG
                     VADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      1811..2063
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2471..2815
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    2824..2862
                     /label=polylinker
                     /note="polylinker"
     terminator      2875..3075
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti
                     plasmid gene 7"
     misc_feature    complement(3076..3601)
                     /label=3'UTR of T-DNA gene 5
                     /note="3'UTR of T-DNA gene 5"
     misc_feature    complement(3678..3702)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     oriT            4257..4366
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             4399..4767
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     CDS             5042..6187
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(7466..8662)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             8768..9415
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     rep_origin      complement(10413..11122)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"