pSB1 vector (V010817)

LOCUS       V010817                36909 bp    DNA     circular UNA 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V010817
VERSION     V010817
KEYWORDS    pSB1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 36909)
  AUTHORS   Komari T, Hiei Y, Saito Y, Murai N, Kumashiro T.
  TITLE     Vectors carrying two separate T-DNAs for co-transformation of higher
            plants mediated by Agrobacterium tumefaciens and segregation of
            transformants free from selection markers.
  JOURNAL   Plant J. 1996;10:165-74.
   PUBMED   8758986
REFERENCE   2  (bases 1 to 36909)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 36909)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.";
            date: "1996"; volume: "10"; pages: "165-74"
            SGRef: number: 2; type: "Journal Article"
            Homologous recombination with the intermediate vector occurs in the
            region spanning the cos and bom sites.
FEATURES             Location/Qualifiers
     source          1..36909
                     /mol_type="genomic DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(353..796)
                     /codon_start=1
                     /product="VirD1"
                     /note="virD1"
                     /note="cleavage accessory protein"
                     /translation="MSKHTRATSSETTINQHRSLKVEGFKVVSARLRSAEYETFSYQAR
                     LLGLSDSMAIRVAVRRIGGFLEIDADTREKMEAILQSIGILSSNVSMLLSAYAEDPRSD
                     LEAVRDERIAFGEAFAALDGLLRSILSVSRRRIDGRSLLKGAL"
     CDS             1065..1759
                     /product="VirC1, inactivated by a frameshift mutation that
                     eliminated the final C in codon Thr-56"
                     /note="virC1 (inactivated)"
     CDS             1762..2370
                     /codon_start=1
                     /product="VirC2"
                     /note="virC2"
                     /translation="MAIRKPALSVGEARRLAGARPEIHHPNPTLVPQKLDLQHLPEKAD
                     EKDQQREPLVADHIYSPDRQLKLTVDALSPPPSPKKLQVFLSARPPAPQVSKTYDNLVR
                     QYSPSKSLQMILRRALDDFESMLADGSFRVAPKSYPIPSTTEKSVLVQTSRMFPVALLE
                     VARSHFDPLGLETARAFGHKLATAALASFFAGEKPSSNW"
     CDS             complement(2484..3284)
                     /gene="virG"
                     /label="Regulatory protein VirG"
                     /note="Regulatory protein VirG from Agrobacterium
                     tumefaciens (strain 15955). Accession#: P62722"
     CDS             complement(3419..4447)
                     /gene="virB11"
                     /label="Protein VirB11"
                     /note="Protein VirB11 from Rhizobium radiobacter.
                     Accession#: P0A3F8"
     CDS             complement(4487..5620)
                     /codon_start=1
                     /product="VirB10"
                     /note="virB10"
                     /note="T-DNA transfer"
                     /translation="MNNDSQQAAHEVDASGSLVSDKHRRRLSGSQKLIVGGVVLALSLS
                     LIWLGGRQKKVNENASPSTLIATNTKPFHPAPIEVPPDPPAVQEAVQPAAPLPPRGEPE
                     RHEPRPEETPIFAYSSGDQGVSKRAIQGDTGRRQEGKRDDNSLPNGEVSGENDLSIRMK
                     PTELQPSSATLLPHPDFMVTQGTIIPCILQTAIDTNLAGYVKCVLPQDIRGTTNNIVLL
                     DRGTTVVGEIQRGLQQGDGRVFVLWDRAETPDHAMISLTSPSADELGRSGLPGSVDSHF
                     WQRFSGAMLLSVVQGAFQAASTYAGSSGGGMSFNSFQNNGEQTTETALKATINIPPTLK
                     KNQGDTVSIFVARDLDFFGVYQLRLTGGATRGRNRRS"
     CDS             complement(5620..6498)
                     /gene="virB9"
                     /label="Protein virB9"
                     /note="Protein virB9 from Rhizobium radiobacter.
                     Accession#: P0A3W6"
     CDS             complement(6495..7208)
                     /codon_start=1
                     /product="VirB8"
                     /note="virB8"
                     /note="T-DNA transfer"
                     /translation="MTGPEYAMLVARESLAEHYKEVEAFQTARAKSARRLSKLIAAVAA
                     IAILGNVAQAFAIATMVPLSRLVPVYLWIRPDGTVDSEVSVSRLPATQEEAVVNASLWE
                     YVRLRESYDADTAQYAYDLVSNFSAPTVRQDYQQFFNYPNPSSPQVILGKRGRVEVEHI
                     ASNDVTPSTQQIRYKRTLVVDGKMPVVSTWTATVRYEKVTSLPGRLRLTNPAGLVVTSY
                     QTSEDTVSNVGHSEP"
     CDS             complement(7198..7362)
                     /gene="virB7"
                     /label="Outer membrane lipoprotein virB7"
                     /note="Outer membrane lipoprotein virB7 from Rhizobium
                     radiobacter. Accession#: P0A3W4"
     CDS             complement(7396..8283)
                     /codon_start=1
                     /product="VirB6"
                     /note="virB6"
                     /note="T-DNA transfer"
                     /translation="MNFTIPAPFTAIHTIFDVAFTTGLDSMLETIQEAVSAPLIACVTL
                     WIIVQGILVIRGEVDTRSGITRVITVTIVVALIVGQANYQDYVVSIFEKTVPNFVQQFS
                     VTGLPLQTVPAQLDTMFAVTQAVFQKIASEIGPMNDQDILAFQGAQWVLYGTLWSAFGV
                     YDAVGILTKVLLAIGPLILVGYIFDRTRDIAAKWIGQLITYGLLLLLLNLVATIVILTE
                     ATALTLMLGVITFAGTTAAKIIGLYELDMFFLTGDALIVALPAIAGNIGGSYWSGATQS
                     ASSLYRRFAQVERG"
     CDS             complement(8380..9042)
                     /codon_start=1
                     /product="VirB5"
                     /note="virB5"
                     /note="T-DNA transfer"
                     /translation="MKTTQLIATVLTCSFLYIQPARAQFVVSDPATEAETLATALATAE
                     NLTQTIAMVTMLTSAYGVTGLLTSLNQKNQYPSTKDLDNEMFSPRMPMSTTARAITSDT
                     DRAVVGSDAEADLLRSQITGSANSAGIAADNLETMDKRLTANADTSAQLSRSRNIMQAT
                     VTNGLLLKQIHDAMIQNVQATSLLTMTTAQAGLHEAEEAAAQRKEHQKTAVIFGALP"
     CDS             complement(9057..11426)
                     /codon_start=1
                     /product="VirB4"
                     /note="virB4"
                     /note="integral inner membrane ATPase"
                     /translation="MLGASGTTERSGEIYLPYIGHLSDHIVLLEDGSIMTIARIDGVAF
                     ELEETEMRNARCRAFNTLLRNIADDHVSIYAHLVRHADVPSSAPRHFRSVFAASLNEAF
                     EQRVLSGQLLRNEHFLTLIVYPQAALGKVKRRFTKLSGKRENDLTGQIRNMEDLWHVVA
                     GSLKAYGLHRLGIREKQGVLFTEIGEALRLIMTGRFTPVPVVSGSLGASIYTDRVICGK
                     RGLEIRTPKDSYVGSIYSFREYPAKTRPGMLNALLSLDFPLVLTQSFSFLTRPQAHAKL
                     SLKSSQMLSSGDKAVTQIGKLSEAEDALASNEFVMGSHHLSLCVYADDLNSLGDRGARA
                     RTRMADAGAVVVQEGIGMEAAYWSQLPGNFKWRTRPGAITSRNFAGFVSFENFPEGASS
                     GHWGNAIARFRTNGGTPFDYIPHEHDVGMTAIFGPIGRGKTTLMMFVLAMLEQSMVDRA
                     GTVVFFDKDRGGELLVRATGGTYLALHRGTPSGLAPLRGLENTAASHDFLREWIVALIE
                     SDGRGGISPEENRRLVRGIHRQLSFDPQMRSIAGLREFLLHGPAEGAGARLQRWCRGHA
                     LGWAFDGEVDEVKLDPSITGFDMTHLLEYEEVCAPAAAYLLHRIGAMIDGRRFVMSCDE
                     FRAYLLNPKFSTVVDKFLLTVRKNNGMLILATQQPEHVLESPLGASLVAQCMTKIFYPS
                     PTADRSAYVDGLKCTEKEFQAIREDMTVGSRKFLLKRESGSVICEFDLRDMREYVAVLS
                     GRANTVRFATRLREAQEGNSSGWLSEFMARHHEAED"
     CDS             complement(11426..11752)
                     /codon_start=1
                     /product="VirB3"
                     /note="virB3"
                     /note="T-DNA transfer"
                     /translation="MADRLEESTLYLAATRPALFLGVPLTLAGLFMMFAGFVIVIVQNP
                     LYEVVLVPLWFAARLIVERDYNAASVVLLFLRTAGRSIDSAVWGGATVSPNPIRVPPRG
                     RGMV"
     CDS             complement(11755..12117)
                     /gene="virB2"
                     /label="Protein virB2"
                     /note="Protein virB2 from Rhizobium radiobacter.
                     Accession#: P09776"
     CDS             complement(12132..12851)
                     /codon_start=1
                     /product="VirB1"
                     /note="virB1"
                     /note="T-DNA transfer"
                     /translation="MLKRSGSLSLALMVSFCSSSLATPLSSAEFDHVARKCAPSVATST
                     LAAIAKVESRFDPLAIHDNTTGETLHWQDHSQATQVVRHRLDARHSLDVGLMQINSRNF
                     SMLGLTPDGALQACTSLSAAANMLKSRYAGGETIDEKQFALRRAISAYNTGNFIGGFAN
                     GYVRKVETAAQSLVPALIEPPKDDHEALKSEETWDVWGSYQRRSQEDGAGGLIAPPPPH
                     QDNGKSADDNQVLFDLY"
     misc_feature    15010..15150
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(15336..15924)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    16432..16830
                     /label="cos"
                     /note="lambda cos site; allows packaging into phage lambda
                     particles"
     promoter        complement(17394..17498)
                     /label="AmpR promoter"
     CDS             complement(18335..18982)
                     /label="TetR"
                     /note="tetracycline resistance regulatory protein"
     CDS             19088..20284
                     /label="TcR"
                     /note="tetracycline efflux protein"
     CDS             complement(21563..22708)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(26121..26489)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(26522..26631)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     operon          complement(28439..30352)
                     /operon="kfrBC"
                     /label="kfrBC operon"
                     /note="kfrBC operon"
     operon          complement(30253..31335)
                     /operon="kfrA"
                     /label="kfrA operon"
                     /note="kfrA operon"
     CDS             complement(32579..33652)
                     /gene="korB"
                     /label="Transcriptional repressor protein KorB"
                     /note="Transcriptional repressor protein KorB from
                     Escherichia coli. Accession#: P07674"
     rep_origin      complement(35754..36465)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"