pQE-30 Xa vector (V010795)

LOCUS       pQE-30_Xa.        3509 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expressing N-terminally 6xHis-tagged proteins 
            with a Factor Xa cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-30 Xa
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Clone at StuI to insert a coding sequence just after the Factor Xa 
            cleavage site.
FEATURES             Location/Qualifiers
     source          1..3509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             166..177
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     misc_feature    240..250
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      256..350
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             394..1050
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1118..1204
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1359..1499
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1685..2273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2447..3304)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3305..3409)
                     /label=AmpR promoter