pQE-81L vector (V010775)

LOCUS       pQE-81L.        4753 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial lacIq vector for expressing N-terminally 6xHis-tagged 
            proteins. For other reading frames, use pQE-80L or pQE-82L.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-81L
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Because this vector contains the lacIq gene, the pREP4 plasmid is 
            not needed.
FEATURES             Location/Qualifiers
     source          1..4753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    147..194
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    194..204
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      210..304
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             348..1004
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1072..1158
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    complement(1217..1238)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1254..2333)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(2334..2411)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    2603..2743
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2929..3517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3691..4548)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4549..4653)
                     /label=AmpR promoter