pMCSG43 vector (V010655)

LOCUS       V010655                 6773 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V010655
VERSION     V010655
KEYWORDS    pMCSG43
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Midwest Center for Structural Genomics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
            For ligation-independent cloning (LIC), linearize with SmaI and
            treat with T4 DNA polymerase plus dATP.
FEATURES             Location/Qualifiers
     source          1..6773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(140..157)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(208..225)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(232..252)
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             complement(263..283)
                     /label="TVMV site"
                     /note="tobacco vein mottling virus (TVMV) NIa protease
                     recognition and cleavage site"
     CDS             complement(305..907)
                     /gene="osmY"
                     /label="Osmotically-inducible protein Y"
                     /note="Osmotically-inducible protein Y from Escherichia
                     coli (strain K12). Accession#: P0AFH8"
     RBS             complement(915..937)
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(952..976)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(977..995)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1213..1444
                     /label="dsbA promoter"
                     /note="promoter for the E. coli dsbA gene (Belin and
                     Boquet, 1994)"
     CDS             1445..2068
                     /label="DsbA"
                     /note="bacterial periplasmic oxidoreductase"
     promoter        2176..2253
                     /label="lacI promoter"
     CDS             2254..3333
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    3349..3370
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             4145..4333
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    4438..4580
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4766..5354)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5528..6385)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6386..6489)
                     /label="AmpR promoter"