Basic Vector Information
- Vector Name:
- pLenti-EF1a-C-mGFP
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 7612 bp
- Type:
- Viral Expression & Packaging Vectors
- Replication origin:
- ori
- Source/Author:
- OriGene
- Copy Number:
- High copy number
- Promoter:
- EF-1α
pLenti-EF1a-C-mGFP vector Map
pLenti-EF1a-C-mGFP vector Sequence
LOCUS V010473 7612 bp DNA circular SYN 01-JAN-1980 DEFINITION Exported. ACCESSION V010473 VERSION V010473 KEYWORDS pLenti-EF1a-C-mGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 7612) AUTHORS OriGene TITLE Direct Submission REFERENCE 2 (bases 1 to 7612) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" Inserts are typically cloned between the SgfI (AsiSI) and MluI sites. FEATURES Location/Qualifiers source 1..7612 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 237..616 /label="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 617..819 /label="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" LTR 834..1014 /label="5' LTR (truncated)" /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1061..1186 /label="HIV-1 Psi" /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1683..1916 /label="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2101..2145 /label="gp41 peptide" /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 2294..2335 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" /label="Protein Tat" misc_feature 2443..2560 /label="cPPT/CTS" /note="central polypurine tract and central termination sequence of HIV-1" promoter 2624..3802 /label="EF-1-alpha promoter" /note="strong constitutive promoter for human elongation factor EF-1-alpha" misc_feature 3818..3921 /label="MCS" /note="MCS" /note="multiple cloning site" CDS 3927..4640 /label="TagGFP2" /note="monomeric green fluorescent protein, also known as mTagGFP" protein_bind complement(4707..4740) /label="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." misc_feature 4796..5384 /label="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5387..5403) /label="KS primer" /note="common sequencing primer, one of multiple similar variants" LTR 5913..6093 /label="3' LTR (truncated)" /note="3' LTR (truncated)" /note="truncated 3' long terminal repeat (LTR) from HIV-1" rep_origin complement(6155..6743) /direction=LEFT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6823..7479) /label="CmR" /note="chloramphenicol acetyltransferase" promoter complement(7480..7584) /label="AmpR promoter"
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