pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro vector (V010403)

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LOCUS       pMSCV16URP-U6-sh        7602 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Lentiviral vector derived from murine embryonic stem cell virus, for
            barcoded shRNA libraries, with TagRFP and puromycin resistance 
            markers.
ACCESSION   .
VERSION     .
KEYWORDS    pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Cellecta
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7602)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..515
                     /label=MSCV
                     /note="Murine embryonic stem cell virus promoter including
                     the enhancer and promoter region of PCMV virus and 5' 
                     untranslated region of dl-587rev retrovirus"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     promoter        1438..1678
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    1684..1713
                     /label=cloning site
                     /note="cloning site"
                     /note="shRNA cloning site"
     promoter        1872..2271
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     CDS             2284..2994
                     /label=TagRFP
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
     CDS             3001..3054
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=2A peptide from Thosea asigna virus capsid
                     protein
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3061..3657
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     LTR             3736..4161
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     promoter        4427..4756
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(5049..5637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5811..6668)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6669..6773)
                     /label=AmpR promoter
     LTR             7601..7602
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem
                     cell virus"