pMSCVhyg vector (V010402)

LOCUS       pMSCVhyg.        6945 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Retroviral vector with a hygromycin resistance marker optimized for 
            gene expression in hematopoietic, embryonic stem, or embryonic 
            carcinoma cells.
ACCESSION   .
VERSION     .
KEYWORDS    pMSCVhyg
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..515
                     /label=MSCV
                     /note="Murine embryonic stem cell virus promoter including
                     the enhancer and promoter region of PCMV virus and 5' 
                     untranslated region of dl-587rev retrovirus"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1410..1433
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        1438..1937
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             1955..2977
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     LTR             3338..3852
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem
                     cell virus"
     protein_bind    complement(4014..4030)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4038..4068)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4083..4104)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4392..4980)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5154..6011)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6012..6116)
                     /label=AmpR promoter
     LTR             6944..6945
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem
                     cell virus"