pRetroX-IRES-DsRedExpress vector (V010387)

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LOCUS       pRetroX-IRES-DsR        5934 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bicistronic retroviral vector for expressing a gene together with 
            the fluorescent protein DsRed-Express.
ACCESSION   .
VERSION     .
KEYWORDS    pRetroX-IRES-DsRedExpress
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..592
                     /label=5' LTR
                     /note="5' LTR"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    655..1012
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     intron          1075..1278
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of
                     human EF-1-alpha"
     exon            1279..1308
                     /label=EF-1-alpha exon
                     /note="truncated exon 2 from human EF-1-alpha, beginning 
                     with a GT-to-CC mutation that reduces splicing efficiency"
     misc_feature    1362..1389
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    1393..1972
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             1967..2641
                     /label=DsRed-Express
                     /note="rapidly maturing tetrameric variant of DsRed
                     fluorescent protein (Bevis and Glick, 2002)"
     LTR             2697..3290
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     primer_bind     complement(3306..3322)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3330..3346)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3354..3384)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3399..3420)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3708..4296)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4470..5327)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5328..5432)
                     /label=AmpR promoter
     primer_bind     5906..5922
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"