pRetroX-PTuner IRES vector (V010385)

LOCUS       pRetroX-PTuner_I        6283 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Retroviral vector for fusing a destabilization domain to the 
            N-terminus of a partner protein, with co-expression of ZsGreen1.
ACCESSION   .
VERSION     .
KEYWORDS    pRetroX-PTuner IRES
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    655..1012
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     intron          1075..1278
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of
                     human EF-1-alpha"
     exon            1279..1308
                     /label=EF-1-alpha exon
                     /note="truncated exon 2 from human EF-1-alpha, beginning 
                     with a GT-to-CC mutation that reduces splicing efficiency"
     CDS             1368..1691
                     /label=DD
                     /note="destabilization domain that can be stabilized by
                     Shield1 in the ProteoTuner(TM) system"
     misc_feature    1694..1719
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    1723..2302
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             2297..2989
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
     LTR             3046..3639
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     primer_bind     complement(3655..3671)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3679..3695)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3703..3733)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3748..3769)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4057..4645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4819..5676)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5677..5781)
                     /label=AmpR promoter
     primer_bind     6255..6271
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"