Basic Vector Information
Retroviral vector to be used in conjunction with a Tet-responsive vector for doxycycline-inducible expression of a gene.
- Vector Name:
- pRetroX-Tet3G
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8136 bp
- Type:
- Viral Expression & Packaging Vectors
- Replication origin:
- ori
- Source/Author:
- Clontech
- Selection Marker:
- Neomycin/G418(Geneticin)
- Copy Number:
- High copy number
- Promoter:
- CMV
pRetroX-Tet3G vector Map
pRetroX-Tet3G vector Sequence
LOCUS pRetroX-Tet3G. 8136 bp DNA circular SYN 01-JAN-1980
DEFINITION Retroviral vector to be used in conjunction with a Tet-responsive
vector for doxycycline-inducible expression of a gene.
ACCESSION .
VERSION .
KEYWORDS pRetroX-Tet3G
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8136)
AUTHORS Clontech
TITLE Direct Submission
REFERENCE 2 (bases 1 to 8136)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8136
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 5..308
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 309..512
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 513..688
/label=5' LTR (truncated)
/note="truncated long terminal repeat from Moloney murine
sarcoma virus"
misc_feature 751..950
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1151..1567
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
enhancer 1604..1907
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 1908..2111
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2290..3033
/label=Tet-On(R) 3G
/note="modified rtTA protein that binds tightly to
promoters containing the tet operator in the presence of
doxycycline"
misc_feature 3045..3630
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 3631..4422
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase from Tn5"
LTR 4842..5267
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
Moloney murine leukemia virus"
promoter 5533..5862
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin complement(6155..6743)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6917..7774)
/label=AmpR
/note="beta-lactamase"
promoter complement(7775..7879)
/label=AmpR promoter
enhancer 8065..8136
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
This page is informational only.