pRetroX-Tight-Hyg vector (V010378)

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LOCUS       pRetroX-Tight-Hy        7419 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Retroviral vector for expressing a gene with the Tet-On(R) Advanced 
            or Tet-Off(R) Advanced system.
ACCESSION   .
VERSION     .
KEYWORDS    pRetroX-Tight-Hyg
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7419)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..308
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     promoter        1603..1917
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     misc_feature    1925..1968
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        1973..2472
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             2490..3512
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     LTR             4125..4550
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     promoter        4816..5145
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(5438..6026)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6200..7057)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7058..7162)
                     /label=AmpR promoter
     enhancer        7348..7419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"