pRFP-CB-shLenti vector (V010374)

LOCUS       V010374                 8491 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V010374
VERSION     V010374
KEYWORDS    pRFP-CB-shLenti
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   OriGene
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8491)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
            The shRNA cassette consists of a 29-bp gene-specific sequence,
            followed by a 7-bp loop, followed by the reverse complement of the
            29-bp sequence, followed by a TTTTTT terminator.
FEATURES             Location/Qualifiers
     source          1..8491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        237..616
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..819
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             834..1014
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1683..1916
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2101..2145
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2294..2335
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2443..2560
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2707..2947
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2951..3008
                     /label="shRNA site"
                     /note="shRNA site"
                     /note="shRNA insertion site"
     promoter        3064..3272
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     CDS             3299..3694
                     /label="BSD"
                     /note="blasticidin S deaminase"
     enhancer        4104..4483
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4485..4684
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        4713..4731
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4839..5531
                     /label="TurboRFP"
                     /note="red fluorescent protein from Entacmaea quadricolor"
     protein_bind    complement(5586..5619)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5675..6263
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(6266..6282)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     LTR             6792..6972
                     /label="3' LTR (truncated)"
                     /note="3' LTR (truncated)"
                     /note="truncated 3' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7034..7622)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7702..8358)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(8359..8463)
                     /label="AmpR promoter"