pRS vector (V010373)

LOCUS       pRS.        5432 bp DNA     circular SYN 01-JAN-1980
DEFINITION  HuSH-29 retroviral vector with ampicillin and puromycin resistance 
            markers, for expressing an shRNA from the U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pRS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   OriGene
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The shRNA cassette consists of a 29-bp gene-specific sequence, 
            followed by a 7-bp loop, followed by the reverse complement of the 
            29-bp sequence, followed by a TTTTTT terminator.
FEATURES             Location/Qualifiers
     source          1..5432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        90..330
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    334..385
                     /label=shRNA site
                     /note="shRNA site"
                     /note="shRNA insertion site"
     promoter        441..651
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             673..1269
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     LTR             1351..1944
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(2316..2904)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3085..3942)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3943..4047)
                     /label=AmpR promoter
     LTR             4078..4547
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    4612..4811
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             5004..5420
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"