pTRE3G-Hom1 vector (V010352)

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LOCUS       pTRE3G-Hom1.        3775 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector encoding a doxycycline-inducible FKBP homodimerizer
            domain, for creating soluble fusion proteins that can be dimerized 
            with a drug.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE3G-Hom1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..382
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     misc_feature    383..422
                     /label=5' MCS
                     /note="5' MCS"
                     /note="multiple cloning site upstream of DmrB"
     CDS             423..425
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             435..755
                     /label=DmrB
                     /note="F36V mutant of FK506-binding protein FKBP12"
     misc_feature    756..785
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     intron          927..992
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1122..1142
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    1567..1688
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1945..2533)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2707..3564)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3565..3669)
                     /label=AmpR promoter