pAD-GAL4 vector (V010328)

LOCUS       pAD-GAL4.        7620 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Phagemid vector carrying the GAL4 activation domain for yeast 
            two-hybrid analysis.
ACCESSION   .
VERSION     .
KEYWORDS    pAD-GAL4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             422..424
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             452..472
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             488..829
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     misc_feature    833..869
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(872..890)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1277..1464
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(1585..2676)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(2677..3081)
                     /label=LEU2 promoter
     primer_bind     complement(3123..3139)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3147..3163)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3171..3201)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3216..3237)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     enhancer        complement(3405..3596)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      3729..4184
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      complement(4450..5038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5212..6069)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6070..6174)
                     /label=AmpR promoter
     rep_origin      6456..7620
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"