pEG202 vector (V010323)

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LOCUS       pEG202.       10166 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Episomal yeast two-hybrid vector for constitutive expression of LexA
            fused to the N-terminus of a protein. Also known as pLexA.
ACCESSION   .
VERSION     .
KEYWORDS    pEG202
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10166)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        807..1511
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1538..2143
                     /label=LexA
                     /note="E. coli DNA-binding protein that represses genes
                     involved in the SOS response to DNA damage"
     misc_feature    2144..2182
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      2209..2396
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     rep_origin      3128..4470
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(6117..6773)
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     promoter        complement(6774..6961)
                     /label=HIS3 promoter
     promoter        8026..8130
                     /label=AmpR promoter
     CDS             8131..8988
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      9162..9750
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9936..10076)
                     /label=bom
                     /note="basis of mobility region from pBR322"