Basic Vector Information
- Vector Name:
- pEG202
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10166 bp
- Type:
- Yeast Plasmids
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Clontech
- Copy Number:
- High copy number
- Promoter:
- ADH1(long)
pEG202 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pEG202 vector Sequence
LOCUS pEG202. 10166 bp DNA circular SYN 01-JAN-1980
DEFINITION Episomal yeast two-hybrid vector for constitutive expression of LexA
fused to the N-terminus of a protein. Also known as pLexA.
ACCESSION .
VERSION .
KEYWORDS pEG202.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10166)
AUTHORS Clontech
TITLE Direct Submission
REFERENCE 2 (bases 1 to 10166)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..10166
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 807..1511
/label=ADH1 promoter
/note="promoter for alcohol dehydrogenase 1"
CDS 1538..2143
/label=LexA
/note="E. coli DNA-binding protein that represses genes
involved in the SOS response to DNA damage"
misc_feature 2144..2182
/label=MCS
/note="MCS"
/note="multiple cloning site"
terminator 2209..2396
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
rep_origin 3128..4470
/label=2u ori
/note="yeast 2u plasmid origin of replication"
CDS complement(6117..6773)
/label=HIS3
/note="imidazoleglycerol-phosphate dehydratase, required
for histidine biosynthesis"
promoter complement(6774..6961)
/label=HIS3 promoter
promoter 8026..8130
/label=AmpR promoter
CDS 8131..8988
/label=AmpR
/note="beta-lactamase"
rep_origin 9162..9750
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(9936..10076)
/label=bom
/note="basis of mobility region from pBR322"
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