Basic Vector Information
- Vector Name:
- pEG202
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10166 bp
- Type:
- Yeast Plasmids
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Clontech
- Copy Number:
- High copy number
- Promoter:
- ADH1(long)
pEG202 vector Map
pEG202 vector Sequence
LOCUS pEG202. 10166 bp DNA circular SYN 01-JAN-1980
DEFINITION Episomal yeast two-hybrid vector for constitutive expression of LexA
fused to the N-terminus of a protein. Also known as pLexA.
ACCESSION .
VERSION .
KEYWORDS pEG202
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10166)
AUTHORS Clontech
TITLE Direct Submission
REFERENCE 2 (bases 1 to 10166)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..10166
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 807..1511
/label=ADH1 promoter
/note="promoter for alcohol dehydrogenase 1"
CDS 1538..2143
/label=LexA
/note="E. coli DNA-binding protein that represses genes
involved in the SOS response to DNA damage"
misc_feature 2144..2182
/label=MCS
/note="MCS"
/note="multiple cloning site"
terminator 2209..2396
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
rep_origin 3128..4470
/label=2u ori
/note="yeast 2u plasmid origin of replication"
CDS complement(6117..6773)
/label=HIS3
/note="imidazoleglycerol-phosphate dehydratase, required
for histidine biosynthesis"
promoter complement(6774..6961)
/label=HIS3 promoter
promoter 8026..8130
/label=AmpR promoter
CDS 8131..8988
/label=AmpR
/note="beta-lactamase"
rep_origin 9162..9750
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(9936..10076)
/label=bom
/note="basis of mobility region from pBR322"
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