pFA6a-bleMX6 vector (V010309)

LOCUS       40924_19261        3503 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid for yeast gene deletion using the bleMX6 selectable marker 
            conferring phleomycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Hentges P, Van Driessche B, Tafforeau L, Vandenhaute J, Carr AM.
  TITLE     Three novel antibiotic marker cassettes for gene disruption and 
            marker switching in Schizosaccharomyces pombe.
  JOURNAL   Yeast 2005;22:1013-9.
  PUBMED    16200533
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   EUROSCARF
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3503)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2005"; volume: "22"; pages: "1013-9"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            113..1034
                     /label=bleMX6
                     /note="yeast selectable marker conferring phleomycin
                     resistance"
     promoter        complement(1139..1157)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1415..2003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2177..3034)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3035..3139)
                     /label=AmpR promoter
     promoter        3485..3503
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"