pFA6a-His3MX6-PGAL1-3HA vector (V010302)

LOCUS       pFA6a-His3MX6-PG        4642 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a HIS3MX6 marker for swapping in the GAL1 promoter and 
            adding a triple-HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    pFA6a-His3MX6-PGAL1-3HA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, 
            Philippsen P, Pringle JR.
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1998;14:953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 4642)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4642)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; pages: "953-61"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(50..237)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(270..359)
                     /label=3xHA
                     /note="three tandem HA epitope tags"
     CDS             complement(378..380)
                     /codon_start=1
                     /label=start codon
                     /note="start codon"
                     /translation="M"
     promoter        complement(400..841)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     gene            975..2175
                     /label=HIS3MX6
                     /note="yeast selectable marker encoding the S. pombe his5
                     gene, which corresponds to S. cerevisiae HIS3"
     promoter        complement(2280..2298)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2556..3144)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3318..4175)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4176..4280)
                     /label=AmpR promoter
     promoter        4626..4642
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"