pFLDα vector (V010284)

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LOCUS       pFLD(alpha).        4694 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Pichia pastoris vector for methylamine-inducible expression of a 
            secreted protein.
ACCESSION   .
VERSION     .
KEYWORDS    pFLD(alpha)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Linearize within the FLD1 promoter for integration.
FEATURES             Location/Qualifiers
     source          1..4694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..597
                     /label=FLD1 promoter
                     /note="methanol- and methylamine-inducible promoter for
                     Pichia pastoris formaldehyde dehydrogenase"
     CDS             622..888
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
     misc_feature    889..959
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             964..1005
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             1015..1032
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      1112..1358
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1725..1829
                     /label=AmpR promoter
     CDS             1830..2687
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2861..3449
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3503..3910
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        3917..3964
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             3983..4354
                     /label=BleoR
                     /note="antibiotic-binding protein"
     terminator      4423..4670
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"