pFLD vector (V010283)

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LOCUS       pFLD.        4409 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Pichia pastoris vector for methylamine-inducible intracellular 
            expression of a protein.
ACCESSION   .
VERSION     .
KEYWORDS    pFLD
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Linearize within the FLD1 promoter for integration.
FEATURES             Location/Qualifiers
     source          1..4409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..597
                     /label=FLD1 promoter
                     /note="methanol- and methylamine-inducible promoter for
                     Pichia pastoris formaldehyde dehydrogenase"
     misc_feature    598..674
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             679..720
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             730..747
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      827..1073
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1440..1544
                     /label=AmpR promoter
     CDS             1545..2402
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2576..3164
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3218..3625
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        3632..3679
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             3698..4069
                     /label=BleoR
                     /note="antibiotic-binding protein"
     terminator      4138..4385
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"