pGAD-C3 vector (V010280)

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LOCUS       pGAD-C3.        6666 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast two-hybrid """"prey"""" vector for fusing a gene to the GAL4 
            activation domain. For other reading frames, use pGAD-C1 or pGAD-C2.
ACCESSION   .
VERSION     .
KEYWORDS    pGAD-C3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   James P, Halladay J, Craig EA.
  TITLE     Genomic libraries and a host strain designed for highly efficient 
            two-hybrid selection in yeast.
  JOURNAL   Genetics 1996;144:1425-36.
  PUBMED    8978031
REFERENCE   2  (bases 1 to 6666)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6666)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "1996"; volume: "144"; pages: "1425-36"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             452..472
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             488..829
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     misc_feature    833..873
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      1248..1435
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(1556..2647)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(2648..3052)
                     /label=LEU2 promoter
     primer_bind     complement(3094..3110)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3118..3134)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3142..3172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3187..3208)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3496..4084)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4258..5115)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5116..5220)
                     /label=AmpR promoter
     rep_origin      5502..6666
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"