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pGAPZ C vector (V010274)

Basic Vector Information

Vector Name:
pGAPZ C
Antibiotic Resistance:
Bleomycin
Length:
2883 bp
Type:
Yeast Plasmids
Replication origin:
ori
Host:
Yeast
Source/Author:
Invitrogen (Life Technologies)
Copy Number:
High copy number
Promoter:
GAP

pGAPZ C vector Map

Copy Sequence View Map
pGAPZ C2883 bp600120018002400GAP promoterMyc6xHisAOX1 terminatorTEF1 promoterEM7 promoterBleoRCYC1 terminatorori

pGAPZ C vector Sequence

LOCUS       40924_21115        2883 bp DNA     circular SYN 13-SEP-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2883)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..483
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris
                     glyceraldehyde-3-phosphate dehydrogenase"
     CDS             563..592
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             608..625
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      704..950
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        965..1376
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        1383..1430
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             1449..1820
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      1889..2136
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2211..2799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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