pGAPZα B vector (V010272)

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LOCUS       pGAPZ-B.        3151 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Pichia pastoris vector for constitutive expression of a secreted 
            protein. For other reading frames, use pGAPZ-alpha A or pGAPZ-alpha 
            C.
ACCESSION   .
VERSION     .
KEYWORDS    pGAPZ-B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3151)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3151)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Linearize within the GAP promoter for integration.
FEATURES             Location/Qualifiers
     source          1..3151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..483
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris
                     glyceraldehyde-3-phosphate dehydrogenase"
     CDS             493..759
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
     misc_feature    758..832
                     /label=MCS
                     /note="MCS"
                     /note="MCS;multiple cloning site"
     CDS             831..860
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             876..893
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      972..1218
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1233..1644
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        1651..1698
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             1717..2088
                     /label=BleoR
                     /note="antibiotic-binding protein"
     terminator      2157..2404
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2479..3067)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"