pPIC6α A vector (V010249)

LOCUS       pPIC6-A.        3646 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Pichia pastoris vector for methanol-inducible expression of a 
            secreted protein. For other reading frames, use pPIC6-alpha B or 
            pPIC6-alpha C.
ACCESSION   .
VERSION     .
KEYWORDS    pPIC6-A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3646)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3646)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Linearize with SacI, PmeI, or BstXI for integration at the AOX1 
            locus.
FEATURES             Location/Qualifiers
     source          1..3646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             941..1207
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
     misc_feature    1208..1276
                     /label=MCS
                     /note="MCS"
                     /note="MCS;multiple cloning site"
     CDS             1275..1304
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1320..1337
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      1417..1663
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1678..2091
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2098..2145
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2164..2559
                     /label=BSD
                     /note="blasticidin S deaminase"
     terminator      2656..2903
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2978..3566)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"