Basic Vector Information
- Vector Name:
- pRS405
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5507 bp
- Type:
- Yeast Plasmids
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Sikorski RS, Hieter P.
- Copy Number:
- High copy number
- Promoter:
- LEU2
- 5' Primer:
- M13 fwd
- 3' Primer:
- M13 rev
pRS405 vector Vector Map
pRS405 vector Sequence
LOCUS 40924_37868 5507 bp DNA circular SYN 01-JAN-1980 DEFINITION Yeast integrative vector with a LEU2 marker and an MCS derived from pBLUESCRIPT II. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5507) AUTHORS Sikorski RS, Hieter P. TITLE A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. JOURNAL Genetics 1989;122:19-27. PUBMED 2659436 REFERENCE 2 (bases 1 to 5507) TITLE Direct Submission REFERENCE 3 (bases 1 to 5507) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; date: "1989"; volume: "122"; pages: "19-27" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..5507 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(666..1757) /codon_start=1 /label=LEU2 /note="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" promoter complement(1770..2174) /label=LEU2 promoter rep_origin complement(2474..2929) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 3074..3090 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 3100..3118 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature complement(3127..3234) /label=MCS /note="pBluescript multiple cloning site" promoter complement(3247..3265) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(3286..3302) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(3310..3326) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3334..3364) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(3379..3400) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(3688..4276) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4450..5307) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5308..5412) /label=AmpR promoter
This page is informational only.