pTEF1/Zeo vector (V010215)

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LOCUS       pTEF1_Zeo.        3556 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid encoding a yeast expression cassette for a Zeocin(TM) 
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    pTEF1 Zeo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     This plasmid lacks sequences for selection and replication in yeast.
FEATURES             Location/Qualifiers
     source          1..3556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    1875..1937
                     /label=MCS 1
                     /note="MCS 1"
                     /note="multiple cloning site 1"
     promoter        1937..2344
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2352..2399
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2418..2789
                     /label=BleoR
                     /note="antibiotic-binding protein"
     terminator      2858..3105
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     misc_feature    3111..3156
                     /label=MCS 2
                     /note="MCS 2"
                     /note="multiple cloning site 2"
     primer_bind     complement(3160..3176)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"