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pYC2/CT vector (V010213) Gene synthesis in pYC2/CT backbone

Basic Vector Information

pYC2-CT is a low-copy shuttle plasmid for Saccharomyces cerevisiae. It has CEN6/ARSH4 for single-copy stability, a galactose-inducible (glucose-repressed) GAL1 promoter, a URA3 marker for screening, and C-terminal V5/6×His tags for protein detection/purification. It’s widely used in yeast gene expression control, protein localization and interaction studies.

Vector Name:
pYC2/CT
Antibiotic Resistance:
Ampicillin
Length:
4627 bp
Type:
Yeast Plasmids
Replication origin:
ori
Source/Author:
Invitrogen (Life Technologies)
Copy Number:
High copy number
Promoter:
GAL1
Growth Strain(s):
DH10B

pYC2/CT vector Map

Copy Sequence View Map
pYC2/CT4627 bp600120018002400300036004200GAL1 promoterT7 promoterMCSV5 tag6xHisCYC1 terminatororiAmpRURA3URA3 promoterCEN/ARS

pYC2/CT vector Sequence

LOCUS       pYC2_CT.        4627 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Centromeric vector with a URA3 marker for galactose-inducible 
            expression of C-terminally tagged proteins in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    pYC2 CT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..443
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        475..493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    501..594
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             607..648
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             658..675
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      709..956
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(1204..1792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1966..2823)
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             complement(2922..3722)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(3723..3943)
                     /label=URA3 promoter
     misc_feature    3971..4474
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"

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