Basic Vector Information
- Vector Name:
- pYC2/NT A
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4702 bp
- Type:
- Yeast Plasmids
- Replication origin:
- ori
- Source/Author:
- Invitrogen (Life Technologies)
- Copy Number:
- High copy number
- Promoter:
- GAL1
pYC2/NT A vector Map
pYC2/NT A vector Sequence
LOCUS pYC2_NT_A. 4702 bp DNA circular SYN 01-JAN-1980 DEFINITION Centromeric vector for galactose-inducible expression of N-terminally tagged proteins in S. cerevisiae. For other reading frames, use pYC2/NT B or pYC2/NT C. ACCESSION . VERSION . KEYWORDS pYC2 NT A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4702) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission REFERENCE 2 (bases 1 to 4702) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..4702 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 2..443 /label=GAL1 promoter /note="inducible promoter, regulated by Gal4" promoter 475..493 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 510..512 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 522..539 /label=6xHis /note="6xHis affinity tag" CDS 543..575 /label=T7 tag (gene 10 leader) /note="leader peptide from bacteriophage T7 gene 10" CDS 579..602 /label=Xpress(TM) tag /note="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" misc_feature 602..669 /label=MCS /note="MCS" /note="multiple cloning site" CDS 682..723 /label=V5 tag /note="epitope tag from simian virus 5" CDS 733..750 /label=6xHis /note="6xHis affinity tag" terminator 784..1031 /label=CYC1 terminator /note="transcription terminator for CYC1" rep_origin complement(1279..1867) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2041..2898) /label=AmpR /note="beta-lactamase" CDS complement(2997..3797) /label=URA3 /note="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" promoter complement(3798..4018) /label=URA3 promoter misc_feature 4046..4549 /label=CEN/ARS /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence"
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