Basic Vector Information
- Vector Name:
- pYC6/CT
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4504 bp
- Type:
- Yeast Plasmids
- Replication origin:
- ori
- Source/Author:
- Invitrogen (Life Technologies)
- Copy Number:
- High copy number
- Promoter:
- GAL1
pYC6/CT vector Vector Map
pYC6/CT vector Sequence
LOCUS pYC6_CT. 4504 bp DNA circular SYN 01-JAN-1980 DEFINITION Centromeric vector with a blasticidin resistance marker for galactose-inducible expression of C-terminally tagged proteins in S. cerevisiae. ACCESSION . VERSION . KEYWORDS pYC6 CT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4504) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission REFERENCE 2 (bases 1 to 4504) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..4504 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 2..443 /label=GAL1 promoter /note="inducible promoter, regulated by Gal4" promoter 475..493 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 495..606 /label=MCS /note="MCS" /note="multiple cloning site" CDS 607..648 /label=V5 tag /note="epitope tag from simian virus 5" CDS 658..675 /label=6xHis /note="6xHis affinity tag" terminator 709..956 /label=CYC1 terminator /note="transcription terminator for CYC1" rep_origin complement(1204..1792) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1966..2823) /label=AmpR /note="beta-lactamase" CDS complement(2946..3341) /label=BSD /note="blasticidin S deaminase" promoter complement(3360..3407) /label=EM7 promoter /note="synthetic bacterial promoter" promoter complement(3414..3821) /label=TEF1 promoter /note="promoter for EF-1-alpha" misc_feature 3848..4351 /label=CEN/ARS /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence"
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