pYES2/CT vector (V010207)

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LOCUS       pYES2_CT.        5963 bp DNA     circular SYN 01-JAN-1980
DEFINITION  High-copy episomal vector with a URA3 marker for galactose-inducible
            expression of C-terminally tagged proteins in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    pYES2 CT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..443
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        475..493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    501..594
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             607..648
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             658..675
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      709..956
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(1204..1792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1966..2823)
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             complement(2922..3722)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(3723..3943)
                     /label=URA3 promoter
     rep_origin      complement(4542..5422)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      complement(5433..5946)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"