pYES2/NT B vector (V010205)

LOCUS       pYES2_NT_B.        6039 bp DNA     circular SYN 01-JAN-1980
DEFINITION  High-copy episomal vector for galactose-inducible expression of 
            N-terminally tagged proteins in S. cerevisiae. For other reading 
            frames, use pYES2/NT A or pYES2/NT C.
ACCESSION   .
VERSION     .
KEYWORDS    pYES2 NT B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..443
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        475..493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             510..512
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             522..539
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             543..575
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             579..602
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     misc_feature    602..670
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             683..724
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             734..751
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      785..1032
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(1280..1868)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2042..2899)
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             complement(2998..3798)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(3799..4019)
                     /label=URA3 promoter
     rep_origin      complement(4618..5498)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      complement(5509..6022)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"