pYM26 vector (V010198)

LOCUS       pYM26.        4298 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a TRP1 marker that provides a template for PCR to fuse 
            yeast enhanced GFP (yeGFP) to the C-terminus of a protein of 
            interest.
ACCESSION   .
VERSION     .
KEYWORDS    pYM26
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H, 
            Moreno-Borchart A, Doenges G, Schwob E, Schiebel E, Knop M.
  TITLE     A versatile toolbox for PCR-based tagging of yeast genes: new 
            fluorescent proteins, more markers and promoter substitution 
            cassettes.
  JOURNAL   Yeast 2004;21:947-62.
  PUBMED    15334558
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   EUROSCARF
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4298)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2004"; volume: "21"; pages: "947-62"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             82..795
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     CDS             complement(886..1518)
                     /codon_start=1
                     /gene="Kluyveromyces lactis TRP1"
                     /product="phosphoribosylanthranilate isomerase, required
                     for tryptophan biosynthesis"
                     /label=Kluyveromyces lactis TRP1
                     /note="KlTRP1"
                     /note="K. lactis auxotrophic marker that also works in S. 
                     cerevisiae"
                     /translation="MLVKVCGLQTVEAAKTAVDDGADYLGIICVPGRKRTIDSSVAKGI
                     STAVHQQENVKGTKLVGVFRNQSVDDVLQLYHEYNLDVIQLHGDEDIKEYRSLIPSSIP
                     IIKRFQFPQDCELLLDLYEHVDNVLTLFDSGEGGTGEKLNWSAISSWSASHPEIKFIIA
                     GGLNPDNVSVAINMLPNAIGVDVSGGVETDGIKDLEKVKLFIQQASQ"
     promoter        complement(1936..1954)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2212..2800)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2974..3831)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3832..3936)
                     /label=AmpR promoter
     promoter        4282..4298
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"