Basic Vector Information
- Vector Name:
- pRS424 with TRP1 marker
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5616 bp
- Type:
- Yeast episomal vector
- Replication origin:
- ori
- Source/Author:
- Sikorski RS, Hieter P.
- Promoter:
- TRP1
pRS424 with TRP1 marker vector Vector Map
pRS424 with TRP1 marker vector Sequence
LOCUS 40924_37983 5616 bp DNA circular SYN 18-DEC-2018 DEFINITION Yeast episomal vector pRS424 with TRP1 marker, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5616) AUTHORS Sikorski RS, Hieter P. TITLE A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae JOURNAL Genetics 122 (1), 19-27 (1989) PUBMED 2659436 REFERENCE 2 (bases 1 to 5616) AUTHORS Christianson TW, Sikorski RS, Dante M, Shero JH, Hieter P. TITLE Multifunctional yeast high-copy-number shuttle vectors JOURNAL Gene 110 (1), 119-122 (1992) PUBMED 1544568 REFERENCE 3 (bases 1 to 5616) AUTHORS Stillman DJ. TITLE Direct Submission JOURNAL Submitted (11-NOV-1993) David J. Stillman, Dept. of Cellular, Viral and Molecular Biology, University of Utah Medical Center, Salt Lake City, UT 84132 USA REFERENCE 4 (bases 1 to 5616) TITLE Direct Submission REFERENCE 5 (bases 1 to 5616) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; date: "1989"; volume: "122"; issue: "1"; pages: "19-27" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Gene"; date: "1992"; volume: "110"; issue: "1"; pages: "119-122" COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted (11-NOV-1993) David J. Stillman, Dept. of Cellular, Viral and Molecular Biology, University of Utah Medical Center, Salt Lake City, UT 84132 USA" COMMENT SGRef: number: 4; type: "Journal Article" FEATURES Location/Qualifiers source 1..5616 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 187..467 /label=TRP1 promoter CDS 468..1139 /codon_start=1 /label=TRP1 /note="phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA KK" rep_origin complement(1241..1696) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 1841..1857 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 1867..1885 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature complement(1894..2001) /label=MCS /note="pBluescript multiple cloning site" promoter complement(2014..2032) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(2053..2069) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(2077..2093) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2101..2131) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(2146..2167) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(2455..3043) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3217..4074) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4075..4179) /label=AmpR promoter rep_origin complement(4206..5548) /direction=LEFT /label=2u ori /note="yeast 2u plasmid origin of replication"
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